Keller received her B.A. in physics from Brandeis University in 1957 and continued her studies in theoretical physics at Harvard University graduating with a Ph.D. in 1963. She became interested in molecular biology during a visit to Cold Spring Harbor Laboratory while completing her Ph.D. dissertation. Her subsequent research has focused on the history and philosophy of modern biology and on gender and science.
She is also on the advisory board of FFIPP-USA (Faculty for Israeli-Palestinian Peace-USA), a network of Palestinian, Israeli, and International faculty, and students, working in for an end of the Israeli occupation of Palestinian territories and just peace.