Her early work used materials such as beeswax before moving on large polyurethane pieces in the 1970s and later to gold-leaf, zinc, and aluminum. The validity of much of her work was questioned until the 1980s due to its use of sensuality and physicality.
Like other artists such as Yves Klein, Benglis' mimicked Jackson Pollock's flinging and dripping methods of painting. Works such as Fallen Painting (1968) inform the approach with a feminist perspective. For this work, Benglis smeared Day-Glo paint across the gallery floor invoking "the depravity of the 'fallen' woman" or, from a feminist perspective, a "prone victim of phallic male desire". These brightly colored organic floor pieces were intended to disrupt the male-dominated minimalism movement with their suggestiveness and openness. In 1971, Benglis began to collaborate with Robert Morris, creating Benglis' video Mumble (1972) and Morris' Exchange (1973).